SARS-CoV Spike Antibody

Immunogen: Recombinant SARS-CoV Spike RBD Protein (Catalog#40150-V08B2)
Preparation It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant SARS-CoV Spike RBD Protein. The antibody was produced using recombinant antibody technology.
Ig Type: Mouse IgG1
Clone ID D004
Specificity: SARS-CoV Spike RBD Protein
Has cross-reactivity in ELISA with SARS-CoV-2 (2019-nCoV) Spike S1 Protein (Cat# 40591-V08H).
Formulation: 0.2 μm filtered solution in PBS
Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. PreservativeFree. Avoid repeated freeze-thaw cycles.
Alternative Names: spike
Applications: ELISA, FCM, ICC/IF, IHC-P, IP
  (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
ELISA ELISA: 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in ACE2-overexpressed 293T cells, infected (left) or noninfected (right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked with 10% serum, and incubated with SARS-CoV Spike monoclonal antibody (dilution ratio 1:60) at 37℃ for 1 hour. Then cells were stained with the FITC-conjugated Goat Anti-human IgG secondary antibody (green).