SARS-CoV-2 (2019-nCoV) Spike Protein(RBD, His Tag)

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Gene Name Synonym: coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBD
Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus
Source: 2019-nCoV
Expression Host: Baculovirus-Insect Cells
QC Testing
Purity: >95 % as determined by SDS-PAGE
Bio Activity: Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 10μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, His Tag) (40592-V08B), the EC50 of 2019-nCoV Spike Protein (RBD, His Tag) (40592-V08B) is 35-70 ng/mL
Endotoxin: <1.0 EU per μg of the protein as determined by the LAL method
Predicted N terminal: Arg 319
Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) consists of 234 amino acids and predicts a molecular mass of 26.54 kDa
Formulation: Lyophilized from sterile 20mM PB, 300mM NaCl, pH 7.0, 10% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
Usage Guide
Stability & Storage: Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Avoid repeated freeze-thaw cycles.
Reconstitution: Detailed reconstitution instructions are sent along with the products.