|Gene Name Synonym:||coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBDD|
|Protein Construction:||A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus|
|Expression Host:||HEK293 Cells|
|Purity:||＞90 % as determined by SDS-PAGE|
|Bio Activity：||Measured by its binding ability in a functional ELISA.
Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 10μg/mL
(100μL/well) can bind 2019-nCoV Spike Protein (S1, His tag) (40591-
V08H),the EC50 of 2019-nCoV Spike Protein (S1, His tag) (40591-V08H)
is 200-400 ng/mL.
|Endotoxin:||＜1.0 EU per μg protein as determined by the LAL method.|
|Predicted N terminal:||Val 16|
|Molecular Mass:||The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 681 amino acids and predicts a molecular mass of 76.5 kDa|
|Formulation:||Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
|Stability & Storage:||Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.|
|Avoid repeated freeze-thaw cycles.|
|Reconstitution:||Detailed reconstitution instructions are sent along with the products.|