|Gene Name Synonym:||coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCPCoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBD|
|Protein Construction:||A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S2 ECD) (YP_009724390.1) (Ser686-Pro1213) was expressed with a polyhistidine tag at the C-terminus.|
|Expression Host:||Baculovirus-Insect Cells|
|Purity:||＞90 % as determined by SDS-PAGE|
|Endotoxin:||＜ 1.0 EU per μg protein as determined by the LAL method.|
|Predicted N terminal:||Ser 686|
|Molecular Mass:||The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S2 ECD, His tag) consists of 539 amino acids and predicts a molecular mass of 59.37 kDa.|
|Formulation:||Lyophilized from sterile 20 mM PB, 300 mM NaCl, 10 % glycerol, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
|Stability & Storage:||Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage|
|Avoid repeated freeze-thaw cycles.|
|Reconstitution:||Detailed reconstitution instructions are sent along with the products.|