|Gene Name Synonym:||coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBD|
|Protein Construction:||A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (QHD43416.1) (Val16-Arg685) was expressed with the Fc region of human IgG1 at the C-terminus.|
|Expression Host:||HEK293 Cells|
|Purity:||＞85 % as determined by SDS-PAGE|
|Bio Activity：||Measured by its binding ability in a functional ELISA.
Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well)
can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc
Tag)(40591-V02H), the EC50 of SARS-CoV-2 (2019-nCoV) Spike
Protein (S1 Subunit, Fc Tag) is 25-60 ng/mL.
|Endotoxin:||＜ 1.0 EU per μg protein as determined by the LAL method.|
|Predicted N terminal:||Val 16|
|Molecular Mass:||The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) consists of 908 amino acids and predicts a molecular mass of 101.7 kDa.|
|Formulation:||Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
|Stability & Storage:||Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.|
|Avoid repeated freeze-thaw cycles.|
|Reconstitution:||Detailed reconstitution instructions are sent along with the products|