SARS-CoV-2 (2019-nCoV) Plpro / papain-like protease (aa 1564-1880, His Tag)

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Gene Name Synonym: Plpro, papain-like protease
Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) papain-like protease (YP_009725299.1) (Glu1564-Val1880) was expressed with a polyhistidine tag at the N-terminus.
Source: 2019-nCoV
Expression Host: E. coli
QC Testing
Purity: >90 % as determined by SDS-PAGE
Bio Activity: Measured by its ability to cleave a fluorogenic peptide substrate, ArgLeu-Arg-Gly-Gly-AMC (RLRGGAMC) (Bachem, Catalog #I-1690). The Specific Activity is >80pmols/min/μg.
Endotoxin: Please contact us for more information.
Predicted N terminal: Met
Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Plpro / papain-like protease (His Tag) consists of 324 amino acids and predicts a molecular mass of 36.79 kDa.
Formulation: Lyophilized from sterile 20mM Tris 500mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
Usage Guide
Stability & Storage: Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Avoid repeated freeze-thaw cycles.
Reconstitution: Detailed reconstitution instructions are sent along with the products.