|Gene Name Synonym:||Plpro, papain-like protease|
|Protein Construction:||A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) papain-like protease (YP_009725299.1) (Glu1564-Val1880) was expressed with a polyhistidine tag at the N-terminus.|
|Expression Host:||E. coli|
|Purity:||＞90 % as determined by SDS-PAGE|
|Bio Activity：||Measured by its ability to cleave a fluorogenic peptide substrate, ArgLeu-Arg-Gly-Gly-AMC (RLRGGAMC) (Bachem, Catalog #I-1690). The Specific Activity is ＞80pmols/min/μg.|
|Endotoxin:||Please contact us for more information.|
|Predicted N terminal:||Met|
|Molecular Mass:||The recombinant SARS-CoV-2 (2019-nCoV) Plpro / papain-like protease (His Tag) consists of 324 amino acids and predicts a molecular mass of 36.79 kDa.|
|Formulation:||Lyophilized from sterile 20mM Tris 500mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
|Stability & Storage:||Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.|
|Avoid repeated freeze-thaw cycles.|
|Reconstitution:||Detailed reconstitution instructions are sent along with the products.|