|Gene Name Synonym:||coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBD|
|Protein Construction:||A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (QHD43416.1) (Arg319-Phe541) was expressed with the Fc region of rabbit IgG1 at the C-terminus.|
|Expression Host:||HEK293 Cells|
|Purity:||＞95 % as determined by SDS-PAGE|
|Bio Activity：||Measured by its binding ability in a functional ELISA.
Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well)
can bind 2019-nCoV Spike Protein (RBD, rabbitFc Tag) (40592-
V31H), the EC50 of 2019-nCoV Spike Protein (RBD, rabbitFc Tag) is
|Endotoxin:||＜1.0 EU per μg of the protein as determined by the LAL method|
|Predicted N terminal:||Arg 319|
|Molecular Mass:||The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) consists of 447 amino acids and predicts a molecular mass of 50.3 kDa.|
|Formulation:||Lyophilized from sterile PBS, PH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
|Stability & Storage:||Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.|
|Avoid repeated freeze-thaw cycles.|
|Reconstitution:||Detailed reconstitution instructions are sent along with the products.|