SARS-CoV-2 (2019-nCoV) Spike Protein(S1+S2 ECD, His tag)

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Gene Name Synonym: coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike;NCP-CoV RBD; NCP-CoV s1; NCP-CoV s2; NCP-CoV Spike; novel coronavirus RBD; novel coronavirus s1; novel coronavirus s2; novel coronavirus spike; RBD; S1; S2; Spike RBD
Protein Construction: A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Val16-Pro1213) was expressed with a polyhistidine tag at the C-terminus.
Source: 2019-nCoV
Expression Host: Baculovirus-Insect Cells
QC Testing
Purity: >90 % as determined by SDS-PAGE
Bio Activity: Immobilized Spike Protein (S1+S2 ECD, His tag) (40589-V08B1) at 156.5-10000ng/mL (100 μL/well) can bind 10ug/ml of Human ACE2, Fc Tag.
Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
Predicted N terminal: Val 16
Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag) consists of 1209 amino acids and predicts a molecular mass of 134.36 kDa.
Formulation: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements
Usage Guide
Stability & Storage: Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Avoid repeated freeze-thaw cycles.
Reconstitution: Detailed reconstitution instructions are sent along with the products.